Research ArticleAsian Journal of Biological and Life SciencesVol. 1 | Issue 2 | 2012 | pp. 96–100Open access
Immobilization of Vigna Mungo β-amylase onto NaCl and NaNO Treated Woven Bombyx Mori Silk Fabrics
- 1*
- 1 Assistant Professor II, Amity Institute of Biotechnology, Amity University, Sec-125, Gautam Buddha Nagar, Noida-201303 (UP), INDIA.
Published in Asian Journal of Biological and Life Sciences
Correspondence: Kirti Rani Sharma
Assistant Professor II, Amity Institute of Biotechnology, Amity University, Sec-125, Gautam Buddha Nagar, Noida-201303 (UP), INDIA.
Email: krsharma@amity.edu
Copyright: © 2012 Manuscript Technomedia. This is an open access article.
- Published:
- Sep 10, 2012
- Received:
- Jun 14, 2012
- Accepted:
- Jun 16, 2012
How to cite
Sharma, K. R. (2012). Immobilization of Vigna Mungo β-amylase onto NaCl and NaNO Treated Woven Bombyx Mori Silk Fabrics. Asian Journal of Biological and Life Sciences, 1(2), 96–100.
Abstract
Vigna Mungo β-amylase was immobilized onto activated Bombyx mori silk fabric by enrichment with chlorination and treated with sodium nitrate (NaNO3 ) via glutaraldehyde coupling. The immobilization of Vigna Mungo β-amylase onto activated Bombyx mori silk fabrics was excellent by having 72-84% of retention of enzyme activity. The immobilization optimum conditions such as pH, reaction time, substrate concentration, CaCl2 concentration and temperature were studied. Thermal stability of the enzyme was improved after immobilization which was 72°C as compared to free enzyme (40°C). The optimum substrate concentration and effect of CaCl2 concentration was also carried out. In addition, the immobilized enzymes had good storage stability and reusability by maintaining 60-70% of its activity after 90-120 days
Subject
Article metadata
| Title | Immobilization of Vigna Mungo β-amylase onto NaCl and NaNO Treated Woven Bombyx Mori Silk Fabrics |
|---|---|
| Authors | Kirti Rani Sharma |
| Affiliations | Assistant Professor II, Amity Institute of Biotechnology, Amity University, Sec-125, Gautam Buddha Nagar, Noida-201303 (UP), INDIA. |
| Corresponding author | krsharma@amity.edu |
| Journal | Asian Journal of Biological and Life Sciences |
| Volume / Issue | Vol. 1, Issue 2 (2012) |
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