Research ArticleAsian Journal of Biological and Life SciencesVol. 1 | Issue 3 | 2012 | pp. 213–218Open access
Nitrogen fixation by the Native Azospirillum spp. Ssolated from Rhizosphere and Non-Rhizosphere of foxtail Millet
- 1,2*,
- 1
- 1 Department of Microbiology, Sri Krishnadevaraya University, Anantapur - 515003, INDIA.
- 2 Department of Biotechnology, Sri Krishnadevaraya University, Anantapur-515003, INDIA.
Published in Asian Journal of Biological and Life Sciences
Correspondence: Mokula Md. Raffi
Department of Microbiology, Sri Krishnadevaraya University, Anantapur - 515003, INDIA.; Department of Biotechnology, Sri Krishnadevaraya University, Anantapur-515003, INDIA.
Email: rafimokula@gmail.com
Copyright: © 2012 Manuscript Technomedia. This is an open access article.
- Published:
- Dec 31, 2012
- Received:
- Jul 13, 2012
- Accepted:
- Aug 11, 2012
How to cite
Raffi, M. M., & Charyulu, P. (2012). Nitrogen fixation by the Native Azospirillum spp. Ssolated from Rhizosphere and Non-Rhizosphere of foxtail Millet. Asian Journal of Biological and Life Sciences, 1(3), 213–218.
Abstract
Experiments were conducted to isolate Azospirillum spp. from the rhizosphere and non-rhizosphere soil of foxtail millet grown in native black loamy soils of Anantapur district. A total of 100 strains of Azospirillum were isolated and grown on semi-solid nitrogen-free bromothymol blue malate semisolid malate agar (Nfb), purified and tested for nitrogen fixing abilities, only 56 strains of Azospirillum spp. strains showed nitrogen fixing ability, -1 out of which 20 strains fixed nitrogen efficiently. Azospirillum strains varied in their nitrogen-fixing abilities ranging from 2.95 to 12.4 mg g malate. All the strains were identified as Azospirillum lipoferum. These strains when tested for IAAproduction, all produced IAA.
Subject
Article metadata
| Title | Nitrogen fixation by the Native Azospirillum spp. Ssolated from Rhizosphere and Non-Rhizosphere of foxtail Millet |
|---|---|
| Authors | Mokula Md. Raffi; P.B.B.N. Charyulu |
| Affiliations | Department of Microbiology, Sri Krishnadevaraya University, Anantapur - 515003, INDIA.; Department of Biotechnology, Sri Krishnadevaraya University, Anantapur-515003, INDIA. |
| Corresponding author | rafimokula@gmail.com |
| Journal | Asian Journal of Biological and Life Sciences |
| Volume / Issue | Vol. 1, Issue 3 (2012) |
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