Antioxidant Studies, in vitro Cytotoxic and Cell Viability Assay of Flavonoids and Alkaloids of Leucas aspera (Wild.) Linn Leaves

Emerging drug advancements for anticancer research is need of the hour using the natural product and their bioactive molecules, among these some are highly potential to fight against cancers and exhibiting as biological agent. The current study was aimed to evaluate the 2, 2-diphenyl-1-picrylhydrazyl, hydroxyl radical, nitric oxide scavenging antioxidant properties, in vitro cytotoxic and cell viability assay of ethanol extracts of the Indian medicinal plant Leucas aspera leaves compounds. The antioxidant property of the ethanol extract was determined two compounds flavonoids and alkaloids and studied for their antioxidant potential by 2, 2-diphenyl-1- picrylhydrazyl (DPPH), hydrogen peroxide radical and nitric oxide free radical scavenging assay and in vitro cytotoxic activity by 3-(4, 5-dimetylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide (MTT) assay using MCF-7 cell lines. The flavonoid compound exhibited maximum antioxidant activity than the alkaloid by comparing the standard gallic acid and also their IC50 concentration. The ethanol extracts of L. aspera leaves exhibited maximum DPPH scavenging property in flavonoid (90%) at 100 μg/mL concentration with an IC50 value 9.25 μg/mL. In cytotoxic assay to determine the % cell viability in MCF-7 cell line also exhibited maximum flavonoids compare to alkaloids, and it has been noticed that dose dependent activity of the compounds. MTT assay IC50 value 247.56 ug/ ml was obtained for MCF-7 for flavonoids and 236.45 ug/ml was obtained for alkaloids compound with optimum effect on % cell viability. The results of this study conclude that Leucas aspera leaves possess the strong antioxidant and anticancer potential which are considered as active therapeutic agents.