Research ArticleAsian Journal of Biological and Life SciencesVol. 10 | Issue 1 | pp. 197–201Open access
Isolation and Identification of Bacterial Strains with Fatty Acid Methyl Ester (FAME) Analysis
- 1,
- 1,
- 1*
- 1 Department of Biochemistry, St. Francis College for Women, Begumpet, Hyderabad, Telangana, INDIA.
Published in Asian Journal of Biological and Life Sciences
Correspondence: Parijatham Kanchana
Department of Biochemistry, St. Francis College for Women, Begumpet, Hyderabad, Telangana, INDIA.
Email: kanchana08@sfc.ac.in
- Received:
- Jan 15, 2021
- Accepted:
- Apr 27, 2021
- DOI:
- 10.5530/ajbls.2021.10.28
How to cite
Kirthi, P. S., Naidu, S., & Kanchana, P. Isolation and Identification of Bacterial Strains with Fatty Acid Methyl Ester (FAME) Analysis. Asian Journal of Biological and Life Sciences, 10(1), 197–201. https://doi.org/10.5530/ajbls.2021.10.28
Abstract
Soil is an excellent source of micro-organisms, especially bacteria. In our present study, we isolated bacteria from a fertile agricultural soil IRCP (361) from Northern India and studied the morphological, cultural, and biochemical characteristics. We performed FAME (Fatty Acid Methyl Ester) analysis of the isolated strains. The isolated strains comprised of Gram- positive bacteria. Using FAME analysis, we confirmed majority of bacteria belong to Bacillus species and few belong to Pseudomonas species. Bacillus species enhance nitrogen fixation which increases soil fertility and protect plants from toxic effects of metals. Pseudomonas confers resistance against rootsecreted antibiotics. Paenibacillus species possess potentiality for bioremediation of xenobiotics. This study shows that the soil is fertile due to the abundance of the Nitrogen fixing bacteria and the identification of the bacteria is fast and accurate using FAME analysis.
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Article metadata
| Title | Isolation and Identification of Bacterial Strains with Fatty Acid Methyl Ester (FAME) Analysis |
|---|---|
| Authors | Pullannagari Sai Kirthi; Srinivasa Naidu; Parijatham Kanchana |
| Affiliations | Department of Biochemistry, St. Francis College for Women, Begumpet, Hyderabad, Telangana, INDIA. |
| Corresponding author | kanchana08@sfc.ac.in |
| Journal | Asian Journal of Biological and Life Sciences |
| Volume / Issue | Vol. 10, Issue 1 |
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