Research ArticleAsian Journal of Biological and Life SciencesVol. 2 | Issue 1 | 2013 | pp. 50–53Open access
The Effects of Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis Venoms on Human Cancer Cells
- 1*,
- 2,
- 1,
- 3,
- 1,
- 1,
- 1
- 1 Research of Development, Queen Saovabha Memorial Institute, Bangkok 10330, THAILAND.
- 2 Cellular Immunology Laboratory Allergy and Clinical Immunology Unit, Department of Medicine, Faculty of Medicine Chulalongkorn, University, Bangkok 10330, THAILAND.
- 3 Institute of Biotechnology and Genetic Engineering, Chulalongkorn University Institute Building 3, Phayathai Road, Patumwan, Bangkok 10330, THAILAND.
Published in Asian Journal of Biological and Life Sciences
Correspondence: Suchitra Khunsap
Research of Development, Queen Saovabha Memorial Institute, Bangkok 10330, THAILAND.
Email: sthaithumnas@yahoo.com
Copyright: © 2013 Manuscript Technomedia. This is an open access article.
- Published:
- Apr 30, 2013
- Received:
- Jan 13, 2013
- Accepted:
- Feb 1, 2013
How to cite
Khunsap, S., Buranapraditkun, S., Suntrarachun, S., Puthong, S., Khow, O., Chulasugandha, P., & Boonchang, S. (2013). The Effects of Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis Venoms on Human Cancer Cells. Asian Journal of Biological and Life Sciences, 2(1), 50–53.
Abstract
Three snake venoms: Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis were studied for cytotoxic activity and apoptosis on five cancer cell lines; BT474, SW620, KATO-III, Hep-G and ChaGo. Cytotoxic effect was determined by MTT assay and flow cytometry has 2 been used for apoptosis. Various concentrations of three venoms showed cytotoxic against cancer cells as time-dependent. The potent of cytotoxic from C. albolabris venom against cancer cells were: BT474 (2.96 ± 0.44 µg/ml), SW620 (3.32 ± 0.14 µg/ml), KATO-III (3.72 ± 0.11 µg/ml) and Hep-G (3.74 ± 0.43 µg/ml), respectively. C. rhodostoma venom showed cytotoxic to BT474 (3.16 ± 0.69 µg/ml), SW620 (3.5 ± 0.01 µg/ml) and KATO¬ 2 III (3.74 ± 0.37 µg/ml) while the venom of D. siamensis was highly toxic to only ChaGo (0.48 ± 0.04 µg/ml). Apoptotic cell death using PI staining was dose and time dependent.
Subject
Article metadata
| Title | The Effects of Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis Venoms on Human Cancer Cells |
|---|---|
| Authors | Suchitra Khunsap; Supranee Buranapraditkun; Sunutcha Suntrarachun; Songchan Puthong; Orawan Khow; Pannipa Chulasugandha; Supatsorn Boonchang |
| Affiliations | Research of Development, Queen Saovabha Memorial Institute, Bangkok 10330, THAILAND.; Cellular Immunology Laboratory Allergy and Clinical Immunology Unit, Department of Medicine, Faculty of Medicine Chulalongkorn, University, Bangkok 10330, THAILAND.; Institute of Biotechnology and Genetic Engineering, Chulalongkorn University Institute Building 3, Phayathai Road, Patumwan, Bangkok 10330, THAILAND. |
| Corresponding author | sthaithumnas@yahoo.com |
| Journal | Asian Journal of Biological and Life Sciences |
| Volume / Issue | Vol. 2, Issue 1 (2013) |
Also in this issue
- Gametogenic Phenology in Freshwater Molluscan Species; Lamellidens Marginalis and Parreysia Corrugata.pp. 1–5
- Lactate Dehydrogenase activity and Isoenzyme Patterns in the Developing Skeletal Muscles of Sialoadenectomised Female Micepp. 6–12
- Antimicrobial activity of Cymbopogon Species against Human Pathogens and Molecular Characterization by RAPD Markerspp. 13–18
- Antioxidant Activities of the Rhizomes of different Zingiberaceae Plants of North-East Indiapp. 19–22
- Estimation of Serum Copper and Magnesium Levels in Diabetic Nephropathy Patientspp. 23–26