Evaluation of Phenolic Compounds and DPPH Inhibition of two Iran Endemic Species from Vincetoxicum Genus

DPPH method is commonly method for determination of antioxidant and it is a free radical. It is possible to determine the antioxidant activity by studying the change of DPPH color. Plant phenols exhibit significant antioxidant properties with redox properties which allow them to act as reducing agents. Vincetoxicum belong to Apocynaceae family has 5 species in Iran and it was used to treat rupture, fever, scrofula and scabies in Chinese medicine. Two species from this genus is endemic to Asia such as Vincetoxicum Pumilum and Vincetoxicum nigrum. In this study, scavenging of DPPH radical and phenolic content of Vincetoxicum Pumilum and Vincetoxicum nigrum was studied. Methanol extracts of plant was prepared by maceration method and then their phenolic contents was evaluated by folin-ciocaltue method and radical scavenging was done with DPPH assay. Amount of phenolic content in Vincetoxicum Pumilum was 11725 µg/g dried extract and it was10150.5 µg/g dried extract for Vincetoxicum nigrum. In all of concentrations, vincetoxicum pumilum had higher power of scavenging the free radicals than vincetoxicum nigrum and vincetoxicum pumilum with high level of phenolic compounds had higher scavenging activity than vincetoxicum nigrum.