Research ArticleAsian Journal of Biological and Life SciencesVol. 5 | Issue 2 | 2016 | pp. 125–130Open access
Development of a Recombinant S1 Domain of the Porcine Epidemic Diarrhea Virus Spike Protein Based ELISA for Virus Detection
- 2*,
- 3*,
- 1*
- 1 Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, TAIWAN.
- 2 Institute of Life Science, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen, VIETNAM.
- 3 Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, Pingtung, TAIWAN.
Published in Asian Journal of Biological and Life Sciences
Correspondence: Nguyen Van Noi
Institute of Life Science, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen, VIETNAM.
Email: nguyenvannoi@tuaf.edu.vn
Copyright: © 2016 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2016
- Received:
- May 14, 2016
- Accepted:
- Aug 13, 2016
How to cite
Noi, N. V., Chung, Y. C., & Cheng, T. C. (2016). Development of a Recombinant S1 Domain of the Porcine Epidemic Diarrhea Virus Spike Protein Based ELISA for Virus Detection. Asian Journal of Biological and Life Sciences, 5(2), 125–130.
Abstract
A recombinant S1 domain of the porcine epidemic diarrhea virus spike protein (S1), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The S1 gene of PEDV was expressed in Escherichia coli. The purified recombinant S1 protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the anti-PEDV-S1 antibody reacted with PEDV-infected cells. The antibody was utilized to develop an indirect ELISA to detect PEDV. Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive.
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Article metadata
| Title | Development of a Recombinant S1 Domain of the Porcine Epidemic Diarrhea Virus Spike Protein Based ELISA for Virus Detection |
|---|---|
| Authors | Nguyen Van Noi; Yao-Chi Chung; Ta-Chih Cheng |
| Affiliations | Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, TAIWAN.; Institute of Life Science, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen, VIETNAM.; Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, Pingtung, TAIWAN. |
| Corresponding author | nguyenvannoi@tuaf.edu.vn |
| Journal | Asian Journal of Biological and Life Sciences |
| Volume / Issue | Vol. 5, Issue 2 (2016) |
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