Extraction and Molecular Characterization of Antimicrobial Metabolites from Streptomyces rochei against Bacterial Leaf Blight of Cotton Caused by Pantoea sp

India ranks among the largest producer of cotton worldwide with high economic value. During the time of cultivation cotton plant normally suffers from the bacterial blight diseases, leading to colour change of affected leaves into light brown exhibiting a blighted appearance. While retrieving the causative agent on nutrient agar plate, yellow pigmented bacteria were consistently recovered which was thought to be caused byXanthomonas oryzaepv. oryzae, the cotton bacterial blight pathogen. However, physiological and molecular analysis of isolated causative agent was of the bacteriumPantoeasp. eighteen strains of active antimicrobial metabolites producingStreptomycessp. were isolated from the soil samples of agricultural field of Mehsana district, North Gujarat, India. All the isolates were assessed for antagonistic activity againstPantoeasp. A causative agent of bacterial blight disease of cotton plant. Among the strains tested, isolate Gray-1 showed strong antimicrobial activity. This isolate was identified as aStreptomyces rocheithrough genetic analysis. The antimicrobial compounds obtained from ethyl acetate extract ofStreptomyces rocheiwere investigated using FTIR and Gas Chromatography-Mass Spectrometry (GC-MS). GC-MS analysis showed the presence 20 bioactive compounds. The most active compounds are 3-Hydroxy-2-Methylbenzaldehyde, 2-Methyl-3-Beta-Furyl propenal, 5-Endo-5-Exo- (Epoxymethano)-6-Methylidene-7 oxabicycloheptane, 4-Hydroxy-Methylbenzaldehyde, 3Hydroxy 4 Methylbenzaldehyde and 3,4 (Methylenedioxy) Toluene, 2-Hydroxy 5 Methylisophthalaldehyde.