Research ArticleAsian Journal of Biological and Life SciencesVol. 9 | Issue 2 | 2020 | pp. 227–233Open access
Effects of Plant Growth Regulators on in vitro Propagation of Economically Important Ornamental Plant Rosa hybrida L.
- *,
- 1,
- 2,
- 1,
- 1,
- 1
- 1 Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana- Ambala, Haryana, INDIA.
- 2 Department of Biotechnology, Multani Mal Modi College, Patiala, Punjab, INDIA.
Published in Asian Journal of Biological and Life Sciences
Correspondence: Diwakar Aggarwal
Email: diwakaraggarwal@yahoo.co.in
Copyright: © 2020 Manuscript Technomedia. This is an open access article.
- Published:
- Aug 30, 2020
- Received:
- Jun 5, 2020
- Accepted:
- Aug 25, 2020
- DOI:
- 10.5530/ajbls.2020.9.35
How to cite
Aggarwal, D., Upadhyay, S. K., Kumar, K., Sehrawat, N., Tuli, H. S., & Singh, R. (2020). Effects of Plant Growth Regulators on in vitro Propagation of Economically Important Ornamental Plant Rosa hybrida L. Asian Journal of Biological and Life Sciences, 9(2), 227–233. https://doi.org/10.5530/ajbls.2020.9.35
Abstract
Micro propagation protocol from selected plants of Rosa hybrida using nodal segments as explant was standardized. Shoot cultures were initiated on MS medium supplemented with 6-benzyle adenine (BA, 2.5 μM). For shoot proliferation, BA and Kinetin (KIN) either alone or in combination with giberellic acid (GA3) were used. BA was found to be better for shoot proliferation and elongation as compared with KIN. Maximum shoot proliferation was observed on MS medium containing 10.0 μM BA in combination with 0.5 μM GA3. Indole 3-butyric acid (IBA) was found to be better auxin for induction of roots and maximum rooting frequency was observed on ¼ MS medium supplemented with 5.0 μM of IBA. Micro propagated plants showed 85 % survival during polyhouse and greenhouse conditions.
Keywords
Subject
Article metadata
| Title | Effects of Plant Growth Regulators on in vitro Propagation of Economically Important Ornamental Plant Rosa hybrida L. |
|---|---|
| Authors | Diwakar Aggarwal; Sushil Kumar Upadhyay; Kuldeep Kumar; Nirmala Sehrawat; Hardeep Singh Tuli; Raj Singh |
| Affiliations | Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana- Ambala, Haryana, INDIA.; Department of Biotechnology, Multani Mal Modi College, Patiala, Punjab, INDIA. |
| Corresponding author | diwakaraggarwal@yahoo.co.in |
| Journal | Asian Journal of Biological and Life Sciences |
| Volume / Issue | Vol. 9, Issue 2 (2020) |
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