Original ArticleIndian Journal of Natural ProductsVol. 36 | Issue 1 | 2022 | pp. 39–43Open access
Biotransformation of Carvedilol Using Plant Cell Culture of Decalepis hamiltonii and Nicotiana tabacum
- 1,
- 1,
- 2,
- 1*
- 1 University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, INDIA.
- 2 Department of Pharmaceutical Sciences, Dr. Harisingh Gour Vishwavidyalaya, Sagar, Madhya Pradesh, INDIA.
Published in Indian Journal of Natural Products
Correspondence: Ciddi Veeresham
University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, INDIA.
Email: ciddiveeresham@yahoo.co.in
Copyright: © 2022 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2022
- DOI:
- 10.5530/ijnp.2022.1.6
How to cite
Shekar, C. R., Rao, A. R., Patil, U. K., & Veeresham, C. (2022). Biotransformation of Carvedilol Using Plant Cell Culture of Decalepis hamiltonii and Nicotiana tabacum. Indian Journal of Natural Products, 36(1), 39–43. https://doi.org/10.5530/ijnp.2022.1.6
Abstract
The biotransformation reaction of cell suspension cultures produced from Decalepis hamiltonii and Nicotiana tabacum leaves were investigated, using Carvedilol drug. Interestingly, the cell suspension cultures showed biotransformation of racemic carvedilol. There is noticeable conversion of S-enantiomer to R-enantiomer of Carvedilol. The bio-conversion was detected and quantified by Chiral HPLC. The ideal biotransformation was achieved with the concentration of 0.1mg/ml Carvedilol at pH 5.8. The optimum incubation time was observed to be 15 days The Decalepis hamiltonii and Nicotiana tabacum cell culture have shown the capacity to convert S-isomer to R-isomer, because it has enzymes specific for conversion from S to R form. Among the two plants Decalepis hamiltonii have shown better biotransformation when compared to Nicotiana tabacum.
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Article metadata
| Title | Biotransformation of Carvedilol Using Plant Cell Culture of Decalepis hamiltonii and Nicotiana tabacum |
|---|---|
| Authors | Ch. Raja Shekar; Ajmera Rama Rao; Umesh K Patil; Ciddi Veeresham |
| Affiliations | University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, INDIA.; Department of Pharmaceutical Sciences, Dr. Harisingh Gour Vishwavidyalaya, Sagar, Madhya Pradesh, INDIA. |
| Corresponding author | ciddiveeresham@yahoo.co.in |
| Journal | Indian Journal of Natural Products |
| Volume / Issue | Vol. 36, Issue 1 (2022) |
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