Bacterial Endophyte Inhabiting Durio zibethinus and its Radical Scavenging and Antidiabetic Potential

Background: Endophytes, markedly receiving interest and has been detected to be remarkable sources of bioactive metabolites. Endophytes, which are also called as endosymbionts have pulled into consideration in the discovery for novel bioactive compounds that can be utilized as new medications. In the current study, endophyte has been isolated from Durio zibethinus leaves. 16s rRNA partial genome sequencing was used to identify selected endophyte and the obtained bacterial endophytic crude extract (DZLM) was subjected for the antioxidant and antidiabetic investigations. Results: Cronobacter sakazakii was identifies as the endophye. Qualitative phytochemical test on DZLM confirmed the presence of phenols, carbohydrates, alkaloids, flavonoids, steroids, mucilage, and glycosides. The antioxidant assay of DZLM showed the good antioxidant potency with IC₅₀±SEM: 161±0.08 µg/mL for DPPH assay and IC₅₀±SEM: 126± 0.09 µg/mL for ABTS assay. Antidiabetic assay results demonstrated a dose-dependent percentage inhibition of the enzymes. The results revealed good inhibition of α-amylase with IC₅₀±SEM: 239±0.08µg/mL and α-glucosidase inhibition with IC₅₀±SEM: 241±0.08 µg/mL for DZLM compared to the standard drug, acarbose. Conclusion: The study uncovered the fact that DZLM has a wellspring of natural source of antioxidant and antidiabetic agents and further studies are required for fractionation of the active constituents existing in DZLM and also their structural elucidation which supports the application of endo-symbionts as the basis of future natural medicines.