Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 56 | Issue 1s | 2022 | pp. S52–S57Open access
In-vitro Evaluation of Anti-microbial and Cytotoxic Activity of Artemisia judaica Leaves and Stem Extracts via Induction of Caspase Dependent Apoptosis
- 1,2,
- 1,
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- 1 College of Pharmacy, Department of Pharmaceutical Chemistry, University of Hail, Hail, SAUDI ARABIA.
- 2 Faculty of Pharmacy, Department of Analytical Chemistry, Cairo University, Cairo, EGYPT.
- 3 College of Pharmacy, Department of Pharmaceutics, University of Hail, Hail, SAUDI ARABIA.
- 4 College of Pharmacy, University of Hail, Hail, SAUDI ARABIA.
- 5 National Organization for Drug Control and Research, Cairo, EGYPT.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Amr Salah Abouzied
College of Pharmacy, Department of Pharmaceutical Chemistry, University of Hail, Hail, SAUDI ARABIA.; National Organization for Drug Control and Research, Cairo, EGYPT.
Email: as.ibrahim@uoh.edu.sa
Copyright: © 2022 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2022
- Received:
- Nov 1, 2021
- Accepted:
- Dec 29, 2021
- DOI:
- 10.5530/ijper.56.1s.42
How to cite
Younes, K. M., Bin, M. K., Unissa, R., Almarshdi, A. A., Alharbi, F. M., Alenzi, S. S., albsher, B. N., & Abouzied, A. S. (2022). In-vitro Evaluation of Anti-microbial and Cytotoxic Activity of Artemisia judaica Leaves and Stem Extracts via Induction of Caspase Dependent Apoptosis. Indian Journal of Pharmaceutical Education and Research, 56(1s), S52–S57. https://doi.org/10.5530/ijper.56.1s.42
Abstract
Medicinal plants and herbs are commonly used in the world to treat various human disorders. Artemisia judaica is one of these herbal species that is commonly used in medicine due to its contents of many bioactive compounds such as; flavonoids, lactones, essential oil and sesqui-terpenoids. It was used in many traditional medicines as an anthelmintic, antispasmodic, anti-rheumatic, and antibacterial agent. In recent years, anti-bacterial and anti-cancer activity of medicinal herbs are highly investigated. The present study is focused on the anti-microbial and cytotoxic effect of methanolic extract of Artemisia judaica leaves and stem. The antimicrobial assay was done on different gram-positive and gram-negative bacteria and it was revealed that leaves and stem extracts possess high and moderate activity against Staphylococcus aureus (MIC 312.5μg/ml and 625μg/ml) and Proteus vulgaris (MIC 312.5μg/ml and 1250μg/ml) for leaves and stem, respectively. Extracts were screened against HepG2, HCT-116, MCF-7, A-549 and MRC-5 cancer cells and it was found that both extracts were active against all cell lines with highest selectivity and cytotoxic activity observed against HepG2 cells (IC50 = 3.38 and 6.84μg/ml, for leaves and stem respectively). Further mechanistic studies on HepG2 cells showed that both extracts resulted in S-phase arrest and induced apoptosis via activation of caspase-3, p53 and Bax
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Article metadata
| Title | In-vitro Evaluation of Anti-microbial and Cytotoxic Activity of Artemisia judaica Leaves and Stem Extracts via Induction of Caspase Dependent Apoptosis |
|---|---|
| Authors | Kareem Mahmoud Younes; Mohamed Khaled Bin; Rahamat Unissa; Afnan Abdulkarem Almarshdi; Fai Mutaz Alharbi; Sulafa Salem Alenzi; Bayan Naef albsher; Amr Salah Abouzied |
| Affiliations | College of Pharmacy, Department of Pharmaceutical Chemistry, University of Hail, Hail, SAUDI ARABIA.; Faculty of Pharmacy, Department of Analytical Chemistry, Cairo University, Cairo, EGYPT.; College of Pharmacy, Department of Pharmaceutics, University of Hail, Hail, SAUDI ARABIA.; College of Pharmacy, University of Hail, Hail, SAUDI ARABIA.; National Organization for Drug Control and Research, Cairo, EGYPT. |
| Corresponding author | as.ibrahim@uoh.edu.sa |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 56, Issue 1s (2022) |
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