research-articleIndian Journal of Pharmaceutical Education and ResearchVol. 58 | Issue 3s | 2024 | pp. s1008–s1017Open access
Exploring the Phytoconstituents, Differential Pharmacology, and Interaction with Empagliflozin of Olea europaea Leaves Extracts
- 1,
- 1*,
- 2,
- 3,
- 3,
- 4,
- 5,
- 6,
- 6,
- 7,
- 8
- 1 Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Department of Pharmaceutics and Pharmaceutical Technology Al-Ahliyya Amman University, Amman, JORDAN.
- 2 Department of Medical Laboratory Sciences, Faculty of Science, Al-Balqa Applied University, Al-Salt, JORDAN.
- 3 Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Petra University, JORDAN.
- 4 Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, Madaba, JORDAN.
- 5 Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Yarmouk University, Irbid, JORDAN.
- 6 Cell Therapy Center, The University of Jordan, Amman, JORDAN.
- 7 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Zarqa University, Zarqa, JORDAN.
- 8 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mutah University, Al-Karak, JORDAN.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Ali Mahmoud Al-Samydai
Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Department of Pharmaceutics and Pharmaceutical Technology Al-Ahliyya Amman University, Amman, JORDAN.
Email: a.alsamydai@ammanu.edu.jo
Copyright: © 2024 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2024
- Received:
- Jun 25, 2023
- Accepted:
- Apr 24, 2024
- DOI:
- 10.5530/ijper.58.3s.100
How to cite
Saeed, Z. K., Al-Samydai, A. M., Alqaraleh, M., Qinna, N. A., Ghanim, B. Y., Mayyas, A., Al-Tawalbeh, D., Alsotari, S. T., Alshaer, W., Al-Mamoori, F., & Al-Halaseh, L. K. (2024). Exploring the Phytoconstituents, Differential Pharmacology, and Interaction with Empagliflozin of Olea europaea Leaves Extracts. Indian Journal of Pharmaceutical Education and Research, 58(3s), s1008–s1017. https://doi.org/10.5530/ijper.58.3s.100
Abstract
Background
The Olea europaea L. leaves have long been used in treating various illnesses. Its pharmacological activity based on the phytoactive constituents; therefore, optimizing the extraction process should intensify the benefits.
Aim
The study aimed to optimize the extraction process for O. europaea L. leaves, evaluate their antioxidant and anti-inflammatory activities, and explore their influence on the biochemical parameters of diabetic animals.
Materials and Methods
The differential pharmacology of the extracts and combinatorial therapy with the antidiabetic agent; empagliflozin, were explored. The aims were accomplished after several in vitro and animal studies: quantification of flavonoid and phenol content, measurement of the antioxidant activity, identification of the active constituents, and assessment of hepatic and renal functions, lipid profile, and glycemic status. In addition, molecular biology tools were used to measure the expression of the inflammatory mediators IL-6 and IL1beta.
Results
Findings reveal that the hydroalcoholic binary system reinforced by the sonication yields the highest polyphenol (44.40±1.414 mg/g dry extract equivalent to gallic acid) and total flavonoids (31.0700±1.202 mg/g dry extract equivalent to quercetin). Extract by the same system showed high substantial antioxidant activity. HPLC-MS/MS reveals oleuropein and its aglycon, o- and p-coumaric acid, hydroxytyrosol acetate, and betaine compounds. A significant reduction in the average weight was recorded in diabetic mice (29.79±2.88 g) compared to the control (32.61 ±2.57 g). A significant reduction in producing the inflammatory mediators IL-6 and IL1 beta was measured.
Conclusion
Olive leaves are a potential addition to conventional medicines to enhance the health profile of diabetic mice.
Keywords
Subject
Article metadata
| Title | Exploring the Phytoconstituents, Differential Pharmacology, and Interaction with Empagliflozin of Olea europaea Leaves Extracts |
|---|---|
| Authors | Zinah Kadhim Saeed; Ali Mahmoud Al-Samydai; Moath Alqaraleh; Nidal Adel Qinna; Bayan Yousef Ghanim; Amal Mayyas; Deniz Al-Tawalbeh; Shrouq Taiseer Alsotari; Walhan Alshaer; Farah Al-Mamoori; Lidia Kamal Al-Halaseh |
| Affiliations | Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Department of Pharmaceutics and Pharmaceutical Technology Al-Ahliyya Amman University, Amman, JORDAN.; Department of Medical Laboratory Sciences, Faculty of Science, Al-Balqa Applied University, Al-Salt, JORDAN.; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Petra University, JORDAN.; Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, Madaba, JORDAN.; Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Yarmouk University, Irbid, JORDAN.; Cell Therapy Center, The University of Jordan, Amman, JORDAN.; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Zarqa University, Zarqa, JORDAN.; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mutah University, Al-Karak, JORDAN. |
| Corresponding author | a.alsamydai@ammanu.edu.jo |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 58, Issue 3s (2024) |
Also in this issue
- Selecting an Appropriate Animal Model for Dyslipidemiapp. s683–s692
- A Review on the in vitro Anticancer Potentials of Acetogenins from Annona muricata Linn. a Potential Inducer of Bax-Bak and Caspase-3 Related Pathwayspp. s693–s703
- Synthesis and Pharmacological Activities of Benzothiazole Derivativespp. s704–s719
- A field Exploration and Inventory of the Most Toxic Plants of Southern Flora Tunisianpp. s720–s731
- A Phytochemicals Approach towards the Role of Dioxins in Disease Progression Targeting Various Pathways: Insightspp. s732–s756