Preclinical Evaluation of Thrombocytopenic Activity of Hydro-Alcoholic Extract of Psidium guajava L. Fruits on Wistar Rats with in silico Modelling on Dengue Virus Protease Inhibitors

Background

The prime objective of the investigation is to measure the capability of expanding the enumeration of platelet in rodent model utilizing hydro-alcoholic extract of Psidium guajava L. fruit in addition with in silico modelling on Dengue Virus Protease Inhibitors.

Materials and Methods

Using a double maceration procedure with 30% water and 70% ethanol, the harvested fruit was extracted. Heparin-induced thrombocytopenic rats were used to assess the extract's thrombocytopenic action. Prednisolone was used as the standard medication and the animals were divided into five categories. The platelet count was performed using a hemocytometer. Additionally, bleeding duration was assessed and at last in silico studies has been carried out through several softwares to identify the targeting molecule.

Results

Prednisolone, Psidium guajava L. low dosage and Psidium guajava L. high dose on the 14^th day demonstrated the numbers of platelet 1732614, 874021 and 946224 respectively compared with toxicant control in the heparin-induced thrombocytopenic rat model.

Conclusion

It is clear from the results above that Psidium guajava L. significantly increased the count of platelet when equated to the toxicant control. Thus, it can be said that Psidium guajava L. plant extract significantly induces thrombocytopenia and it is found that NS2-NB3 protease is the promising target for dengue virus and in future we will try to identify the quercetin or quercetin derivatives of Psidium guajava L. responsible for producing effectiveness against the said target.