Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 59 | Issue 2 | 2025 | pp. 563–573Open access
An Albumin-Glutaraldehyde Nanoparticle Loaded with Berberine Inhibits Cell Growth and Induces Apoptosis in Colon Cancer HCT-116 Cells
- 1*,
- 2*,
- 3*,
- 4*,
- 5*,
- 6*
- 1 Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, INDIA.
- 2 Department of Biochemistry, Centre for Drug Discovery, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, INDIA.
- 3 Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, SAUDI ARABIA.
- 4 Department of Chemistry, Faculty of Sciences, Isra University, Amman, JORDAN.
- 5 Muthayammal Centre for Advanced Research (MCAR), Muthayammal College of Arts and Science, Tamil Nadu, INDIA.
- 6 College of Agricultural and Food Science, Zhejiang Agriculture and Forestry University, Linan, Hangzhou, CHINA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Murugan Alwarkurichi Munusamy
Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, INDIA.
Email: ammurugan11@gmail.com
Copyright: © 2025 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2025
- Received:
- May 27, 2024
- Accepted:
- Dec 7, 2024
- DOI:
- 10.5530/ijper.20257394
How to cite
Munusamy, M. A., Bharathi, M., Alarfaj, A. A., Hussein-Al-Ali, S. H., Sarathbabu, S., & Nagaiya, R. (2025). An Albumin-Glutaraldehyde Nanoparticle Loaded with Berberine Inhibits Cell Growth and Induces Apoptosis in Colon Cancer HCT-116 Cells. Indian Journal of Pharmaceutical Education and Research, 59(2), 563–573. https://doi.org/10.5530/ijper.20257394
Abstract
Background
The study aims to synthesize and characterize berberine-loaded albumin-glutaraldehyde nanoparticles and evaluate their anticancer activity against colon cancer HCT-116 cells. The research focuses on the mechanisms involved in oxidative stress-dependent apoptosis, crucial for inhibiting colon cancer growth and promoting overall health.
Materials and Methods
The synthesized Berberine-Albumin NPs were characterized using several techniques, including UV-visible spectroscopy, X-ray Diffractometer (XRD), Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Fourier Transform Infrared (FT-IR), Dynamic Light Scattering (DLS) and Photoluminescence (PL) studies. The antibacterial potentials of Berberine-Albumin NPs against various pathogens were examined using the well diffusion technique. The cytotoxicity of Berberine-Albumin NPs against HCT-116 cells was evaluated using the MTT assay. The study evaluated ROS production and apoptosis in HCT-116 cells treated with Berberine-Albumin NPs using fluorescence staining and oxidative stress marker levels using assay kits.
Results
The characterization analyses revealed the presence of Berberine-Albumin NPs with a cuboidal shape and an average particle size of 155.80 nm. Several elements and functional groups in Berberine-Albumin NPs were also identified. The synthesized Berberine-Albumin NPs exhibited remarkable antimicrobial properties by efficiently suppressing the growth of several pathogens. The treatment with Berberine-Albumin NPs effectively suppressed the proliferation of HCT-116 cells. The findings of fluorescence staining experiments confirmed more ROS accumulation and apoptosis in HCT-116 cells treated with Berberine-Albumin NPs. The administration of Berberine-Albumin NPs resulted in an upregulation of oxidative stress in HCT-116 cells.
Conclusion
In summary, this work revealed that synthesized Berberine-Albumin NPs had anticancer effects against colon cancer. Hence, the present findings indicate that Berberine-Albumin NPs hold promise as an effective candidate for colon cancer treatment. In future studies, we would like to evaluate clinical relevance, comparative analysis, long-term effects and biodistribution and pharmacokinetics of Berberine-Albumin NPs.
Keywords
Subject
Article metadata
| Title | An Albumin-Glutaraldehyde Nanoparticle Loaded with Berberine Inhibits Cell Growth and Induces Apoptosis in Colon Cancer HCT-116 Cells |
|---|---|
| Authors | Murugan Alwarkurichi Munusamy; Muruganantham Bharathi; Abdullah A. Alarfaj; Samer Hasan Hussein-Al-Ali; Subbarayan Sarathbabu; Ravichandran Nagaiya |
| Affiliations | Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, INDIA.; Department of Biochemistry, Centre for Drug Discovery, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, INDIA.; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, SAUDI ARABIA.; Department of Chemistry, Faculty of Sciences, Isra University, Amman, JORDAN.; Muthayammal Centre for Advanced Research (MCAR), Muthayammal College of Arts and Science, Tamil Nadu, INDIA.; College of Agricultural and Food Science, Zhejiang Agriculture and Forestry University, Linan, Hangzhou, CHINA. |
| Corresponding author | ammurugan11@gmail.com |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 59, Issue 2 (2025) |
Also in this issue
- Pharmacotherapy of Anorexia Nervosa and Bulimia Nervosa Using SSRIs, MAOIs and TCAspp. 425–432
- Fostering Ethical Decision-Making Skills in Pharmaceutical Education: Vital for Future Pharmacistspp. 433–440
- Pro-Inflammatory Cytokines Predict the Response of Cancer Treatmentpp. 441–452
- Deciphering Molecular Insights into Isocitrate Lyase and their Inhibitors: A Detailed Display through Medicinal Chemistry Windowpp. 453–471
- Insights of Nanoemulsion as a Drug Delivery System: An Overview of Current Trends and Applicationspp. 472–492