Calycosin Inhibits Cell Proliferation and Induces Apoptosis in Bladder Cancer T24 Cells

Aim/Background: Calycosin is a primary glycoside derived from the Chinese medicinal plant Radix astragali (RA). The compound has been demonstrated to suppress cell growth and trigger programmed apoptosis in various cancer cell lines. The current investigation was aimed to examine the impact of Calycosin on the proliferation and death of the bladder cancer cell line and its underlying mechanism. Materials and Methods: The present work aimed to investigate the effects of Calycosin on the rate of cell growth, oxidative stress and apoptosis in the T24 human bladder cancer cell line. The viability of Calycosin-treated T24 cells were studied by MTT assay. The apoptosis and ROS production in T24 cells were assessed using fluorescent staining assays. The levels of biochemical markers in the Calycosin-treated T24 cells were examined using kits. Results: Results indicated that exposure to BPs could enhance the generation of Reactive Oxygen Species (ROS) and reduce the levels of Superoxide Dismutase (SOD) and Glutathione (GSH) and elevate the TBA Reactive Species (TBARS). Conclusion: These results indicate that Calycosin may have chemopreventive potential.