Effect of Curcumin and Dioscorea alata L. On Aniline Induced Spleen Toxicity in Rats

Background: In this investigation, the consequences of standardized ethanolic extracts of curcumin and Dioscorea alata L. To assess their therapeutic potential individually and together in spleen poisoning caused by aniline in rats, focusing on hematological, physiological parameters, serum parameters, antioxidant parameter, and histopathological changes over a 30-day period. Materials and Methods: The investigation included six Wistar rats of both sexes per group, weighing between 200-270 g. For 30 days, Water with 100 ppm of AH was administered to rats. To cause splenic damage. The treatment groups were given aniline with Curcumin and Disocorea alata alone and in combination (75 mg/kg/day, orally). Physiological parameters, Hematological parameters, tissue oxidative parameters (GSH, LPO, NO) and serum parameters total protein, total iron were assessed to evaluate treatment effects. Results: Significant Progressions were observed in rats treated with combined Curcumin and Dioscorea alata therapy, these included decrease in body weight of AH-treated rats. Improvements in overall amounts of iron and protein, noteworthy decreases in oxidative stress, significant increases in organ weight of spleen and liver, Hematological analysis showed significant decrease in WBC count, Erythrocyte count and hemoglobin showed significant increase, the spleen always showed notable histological alterations, decreased severity of hemorrhages and aggregation of RBCs in the red and white pulp of spleen tissue. In addition to showing hepatomegaly toxicity, these findings demonstrate a strong link between the combined potential of curcumin and Dioscorea alata in recovering aniline exposure-related splenic lesions and erythrocyte destruction. Conclusion: The study shows the remarkable combined efficacy of curcumin and Dioscorea alata in managing spleen toxicity resulting from exposure to aniline hydrochloride.