Synthesis Characterization of Sulphadiazine Scaffold: In silico and in vitro Antibacterial Activity

Aim: The present study involves the synthesis, characterization, and evaluation of six novel Sulphadiazine derivatives (SV1-SS6) using Schiff base reactions with various aromatic aldehydes. Materials and Methods: The compounds were characterized by melting point analysis, TLC, FTIR, and 1H NMR spectroscopy. In silico studies using Molinspiration, ChemSpider, and Protox-III predicted good drug-likeness, favourable absorption, and low toxicity (Class IV) for all derivatives. Results: Antibacterial activity was assessed against Pseudomonas aeruginosa and Bacillus subtilis using the agar well diffusion method. Among the compounds, SD3 and SS6 showed the most potent activity, particularly against P. aeruginosa, even at lower concentrations. Conclusion: The study highlights the potential of Sulphadiazine derivatives as promising candidates for further development of antibacterial agents.