Phytochemical Screening and Liquid Chromatography-Mass Spectrometry Studies of Ethyl Acetate Extract of Origanum vulgare

Background: Origanum vulgare is a perennial shrub that grows in the mountains of the Mediterranean and Euro/Iran-Siberian regions. Objectives: This study was conducted to identify the major constituents of the ethanolic extract of Origanum vulgare by Liquid chromatography/mass spectroscopy analysis. Methods: The leaves of Origanum vulgare were powdered and the powdered sample of plant material was then subjected to extraction by maceration process for 72 h. The extract obtained was filtered through Whatman filter paper and concentrated under reduced pressure using a rotary vacuum evaporator. Results: The extract contained monoterpene hydrocarbons and phenolic compounds, the major ones being Chlorogenic acid, p-cymene and phytol. Conclusion: Taken together, these results reinforce the importance of this millennial species and open new perspectives for the research and design of new herbal medicines, obtained from indigenous vegetal material.