Formulation, Characterization and Evaluation of Behavioral Effects of Suspension and Effervescent Granules of Evolvulus alsinoides Linn. and Convolvulus pluricaulis Choisy

Objectives: The objective of the present study was to formulate, characterize and evaluate herbal formulations of Evolvulus alsinoides and Convolvulus pluricaulis against scopolamine-induced behavioral disturbances for Alzheimer’s disease. Methods: The herbal drugs were extracted using ethanol. The herbal suspension and effervescence granules were prepared using the extract and evaluated on rats. Scopolamine was used to induce behavioral and locomotor disturbances. Results: Results revealed that both the formulations were stable and effective in enhancing cognition against scopolamine-induced behavioral disturbance. The activity score of suspension of Evolvulus alsinoides and Convolvulus pluricaulis in the actophotometer were 128.19 and 110.23 while in effervescent granules of Evolvulus alsinoides and Convolvulus pluricaulis were 124.21 and 120.29 after 60 min, respectively. Suspension of Evolvulus alsinoides and Convolvulus pluricaulis reduced the fall off time to 94.18 and 102.03 while effervescent granules of Evolvulus alsinoides and Convolvulus pluricaulis reduced 98.01 and 98.16 after 30 min using the rota-rod, respectively. Suspension of Evolvulus alsinoides and Convolvulus pluricaulis reduce the activity of acetylcholinesterase as 19.10 and 18.01 Units/L while effervescent granules of Evolvulus alsinoides and Convolvulus pluricaulis reduce the activity of acetylcholinesterase as 17.69 and 14.36 Units/L. Conclusion: The formulations were significantly reduced locomotor activity using actophotometer, rotarod activity and the level of acetylcholinesterase. Thus, herbal suspension and effervescent granules of Evolvulus alsinoides and Convolvulus pluricaulis may be a safe alternative for Alzheimer’s disease against scopolamine-induced behavioral disturbances.