Validated HPTLC Method to Estimate Silymarin in Marketed Herbal Formulation

Deore, Sharada L; Tidke, Hrutuja P; Khadabadi, Somshekhar S; Baviskar, Bhushan A; Kide, Anajli A; Shende, Bhavna P

doi:10.5530/ijpi.2021.1.10

Original ArticleInternational Journal of Pharmaceutical InvestigationVol. 11 | Issue 1 | pp. 52–55Open access

Validated HPTLC Method to Estimate Silymarin in Marketed Herbal Formulation

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¹ Government College of Pharmacy, Amravati, Maharashtra, INDIA.

Correspondence: Sharada L Deore
Government College of Pharmacy, Amravati, Maharashtra, INDIA.
Email: sharudeore_2@yahoo.com

Received:: Oct 23, 2020
Accepted:: Dec 19, 2020
DOI:: 10.5530/ijpi.2021.1.10

How to cite

Deore, S. L., Tidke, H. P., Khadabadi, S. S., Baviskar, B. A., Kide, A. A., & Shende, B. P. Validated HPTLC Method to Estimate Silymarin in Marketed Herbal Formulation. International Journal of Pharmaceutical Investigation, 11(1), 52–55. https://doi.org/10.5530/ijpi.2021.1.10

Abstract

Objectives: The objective of present research work is to develop simple, rapid and accurate high performance thin layer chromatographic method for quantitative estimation of silymarin in marketed hepatoprotective formulation. Methods: The separation was performed on silica gel 60 F254 precoated TLC aluminum plates and toluene: ethyl acetate: formic acid: methanol as mobile phase. Determination and quantitation were performed by densitometric scanning at 288 nm. Validation of method is carried out as per International Conference of Harmonization (ICH) guidelines. Results: Accuracy, precision and recovery were all within the required limits. Conclusion: In conclusion, the developed HPTLC method is precise and accurate for implementation in routine quality control. The selected marketed hepatoprotective formulation contains the sufficient amount of the active ingredient which is required for its therapeutic efficiency.

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Article metadata

Title	Validated HPTLC Method to Estimate Silymarin in Marketed Herbal Formulation
Authors	Sharada L Deore; Hrutuja P Tidke; Somshekhar S Khadabadi; Bhushan A Baviskar; Anajli A Kide; Bhavna P Shende
Affiliations	Government College of Pharmacy, Amravati, Maharashtra, INDIA.
Corresponding author	sharudeore_2@yahoo.com
Journal	International Journal of Pharmaceutical Investigation
Volume / Issue	Vol. 11, Issue 1

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