Spectroscopic Analysis of Lagos spinach- seed (Celosia argentea Linn.)

Background: Celosia argentea Linn. (C. argentea) commonly known as Cock’s Comb. It’s a leading leafy vegetable used for treatment of anti inflammatory, antioxidant and possessing immunostimulating activities. This species contains a surplus of phytochemicals including terpenoids, glycosides, flavonoids and tannins. The full complement of bioactive chemicals has yet to be identified, which is a critical step in explaining its medical use. The Celosia genus contains around 30 species, the majority of which have had little phytochemical or pharmacological research done on them. The aim of this study is to get a better chemo- semantics of Celosia species, large-scale multi-targeted metabolic profiling and fingerprinting approaches was used. Methods: FTIR, GC-MS and LC-MS spectra of extracted components were analyzed with multivariate data from C. argentea. The crude extract was examined at different wavelengths from 4000 cm-1 and 400 cm-1 by using Shimadzu 8400 Spectrophotometry system and the distinctive peaks were detected. For GC-MS analysis, sample was extracted with methanol (as per API procedure), filtered in ashless filter paper and extract was concentrated. The NIST Ver. 2.0 - Year 2005 library was used for chemical detection. Results: The FTIR profile showed different peaks ranging from 4000 cm-1 and 400 cm-1 and spectrum with confirmed presence of alcohols and phenols, amines, alkanes, alkynes aldehydes and ketones, carboxylic acids functional groups in methanol extract of C. argentea. Analysis of the mass spectrum GC-MS analysis provides different peaks determining presence of Pentadeconic acid, n-Hexadecoic acid, 9-12-Octadecadienoic acid, Cis-vaccenic acid, Octadecanoic acid. The results of LC-MS analysis provide specific data of bioactive components which may suggest the Butralin, Fulvine, Dihydrocapsaicin, Lisinopril, 9Z-Octadecen-12-yonic acid, N-Hexadecanoylpyrrodine etc. The phytoconstituents may use to treat diseases and further insight in future researches on Celosia argentea. Conclusion: The molecules reported through above exploration of spectral techniques may justify traditional therapeutic manifestation of C. argentea and useful in activity guided isolation.