Material in Arabica Coffee Powder (Coffea arabica) with Gel-based PCR Method

Aim/Background: Arabica coffee (Coffea arabica) is the most consumed coffee in the world. As a result, it is susceptible to imitation Arabica coffee made with corn syrup and other inexpensive components (Zea mays). This study detects the corn Zein gene in Arabica coffee powder on the market with the Gel-based PCR method. Materials and Methods: In this study, the samples of research were split into two groups. Samples are separated into two categories: test and control. Controls include unprocessed corn (Zea mays) and coffee beans (Coffea arabica). There are now four distinct varieties of Arabica coffee powder available in the Indonesian market (Coffee arabica). In this study, the simplex PCR method with the primer pair is the Zein gene, and the control primer pair is the ClpP gene as an Arabica coffee gene. Results: The results of this study are that the four test samples show a band parallels to the size of 87 bp (Zein gene band). Conclusion: In conclusion, all test samples (Arabica coffee powder on the market) are suspected of containing the Zein gene, the corn gene.