Original ArticleFree Radicals and AntioxidantsVol. 8 | Issue 2 | pp. 70–73Open access
Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH
- 1,4*,
- 2,4,
- 3,4,
- 4,
- 4,
- 5
- 1 Department of Cardiovascular Surgery, Kaiser Permanente Medical Group, Fontana, California, USA..
- 2 Hunter Holmes McGuire Veterans Ad ministration Medical Centre, Richmond, Virginia, USA..
- 3 Department of Surgery, University of Washington and VA Puget Sounds Healthcare System, Seattle,Washington, USA..
- 4 Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA..
- 5 Director, Center for Epigenetics and Disease Prevention, Texas A and M College of Medicine, Houston, Texas, USA..
Published in Free Radicals and Antioxidants
Correspondence: Nahidh W Hasaniya
Department of Cardiovascular Surgery, Kaiser Permanente Medical Group, Fontana, California, USA..; Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA..
Email: nhasaniya@yahoo.com
- Received:
- Sep 12, 2017
- Accepted:
- May 28, 2018
- DOI:
- 10.5530/fra.2018.2.11
How to cite
Hasaniya, N. W., Premaratne, S., Zhang, W. W., Razzuk, A. M., McNamara, J. J., & Dashwood, R. H. Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH. Free Radicals and Antioxidants, 8(2), 70–73. https://doi.org/10.5530/fra.2018.2.11
Abstract
Objective: Hydrogenation of the stable free radical, 2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl (OXANO•), converts it to the intracellular spin trap, 2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine (OXANOH). Methods: Due to the flammable nature of the reaction, this procedure must be carried out with extreme care. OXANO• (10 mM) was diluted in 50 ml of Krebs-Henseleit buffer (pH 7.4) and hydrogenated in a nitrogen atmosphere in the presence of Platinum (IV) Oxide Hydrate (100 mg). Results: The reaction was completed in approximately 45 min, and was confirmed by the disappearance of the three-electron paramagnetic resonance (EPR) signals (1:1:1). Additionally, the absorption spectra were measured for both OXANOH and OXANO• in the ultraviolet (UV) and visible light regions. Conclusion: OXANOH is an extremely valuable oxygen free radical spin trap due to its ability to trap both extra and intracellular free radicals in biological tissues.
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Article metadata
| Title | Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH |
|---|---|
| Authors | Nahidh W Hasaniya; Shyamal Premaratne; Wayne W Zhang; Aziz M Razzuk; J Judson McNamara; Roderick H Dashwood |
| Affiliations | Department of Cardiovascular Surgery, Kaiser Permanente Medical Group, Fontana, California, USA..; Hunter Holmes McGuire Veterans Ad ministration Medical Centre, Richmond, Virginia, USA..; Department of Surgery, University of Washington and VA Puget Sounds Healthcare System, Seattle,Washington, USA..; Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA..; Director, Center for Epigenetics and Disease Prevention, Texas A and M College of Medicine, Houston, Texas, USA.. |
| Corresponding author | nhasaniya@yahoo.com |
| Journal | Free Radicals and Antioxidants |
| Volume / Issue | Vol. 8, Issue 2 |
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