HPTLC Fingerprinting and Anti-asthmatic Activity of Roots of Two Different Sources of Bharangi

The present research work aims at developing HPTLC fingerprints of two sources of Bharangi Clerodendrum serratum (Linn.) and Clerodendrum indicum (Linn.), along with in vivo antiasthmatic evaluation in OVA-induced Wistar rat model. Air dried roots of both plants were subjected for extraction by maceration followed by soxhlet using ethanol (80%). Further a HPTLC fingerprints was developed for the quantification of Oleanolic acid and Stigma sterol to distinguish both the plants. The anti-asthmatic activity of Clerodendrum serratum and Clerodenrum indicum was evaluated in Ovalbumin induced Wistar rat model and inflammatory parameters like absolute eosinophil count in BALF, total leukocyte count in BALF, absolute eosinophil count in the Blood, IgE antibodies in serum along with the histopathological changes of lungs were studied. HPTLC fingerprinting showed the presence of Oleanolic acid in Clerodendrum serratum and it was found to be absent in Clerodendrum indicum whereas Stigmasterol was found to be present in Clerodendrum indicum and absent in Clerodendrum serratum. In in vivo anti-asthmatic activity, test drugs have shown significant decrease in inflammatory parameters such as Absolute eosinophil count in Blood, Absolute eosinophil count in BALF, Total leukocyte count in the BALF and Concentration of IgE antibodies. Among Extract treated groups CSE1 and CSE2 showed good results with p<0.0001 when compared with asthmatic group. All the studied parameters clearly conclude that both these plants with controversial botanical identity can be distinguished based on their physiochemical and HPTLC fingerprint profiles. The results suggest that the hydroalcoholic extracts of both the plants significantly possess anti-asthmatic activity.