research-articleIndian Journal of Pharmaceutical Education and ResearchVol. 58 | Issue 4 | 2024 | pp. 1373–1379Open access
The Effect of Sophoridine on NSCLC by Down Regulation of mTOR and NOTCH1
- 1,
- 2,3,
- 4,
- 1,
- 5,
- 4,
- 1,6,
- 7,
- 7,
- 1*
- 1 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, SAUDI ARABIA.
- 2 Department of Pathology and Laboratory Medicine, Security Forces Hospital Program, Riyadh, SAUDI ARABIA.
- 3 College of Medicine, Alfaisal University, Riyadh, SAUDI ARABIA.
- 4 Department of Clinical Laboratory Science, College of Applied Medical Science, Taif University, Taif, SAUDI ARABIA.
- 5 Department of Clinical Laboratory Sciences, Turabah University College, Taif University, Taif, SAUDI ARABIA.
- 6 Department of Chemistry (Biochemistry Branch), Faculty of Science, Suez University, Suez, EGYPT.
- 7 Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi, INDIA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Mohammed Ageeli Hakami
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, SAUDI ARABIA.
Email: m.hakami@su.edu.sa
Copyright: © 2024 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2024
- Received:
- Jul 13, 2024
- Accepted:
- Sep 13, 2024
- DOI:
- 10.5530/ijper.58.4.147
How to cite
Hasan, M. R., Hazazi, A., Alsaiari, A. A., Almufarriji, F. M., Albaqami, A., Abdulaziz, O., Darwish, M. K., Kumar, A., Dev, K., & Hakami, M. A. (2024). The Effect of Sophoridine on NSCLC by Down Regulation of mTOR and NOTCH1. Indian Journal of Pharmaceutical Education and Research, 58(4), 1373–1379. https://doi.org/10.5530/ijper.58.4.147
Abstract
Introduction
Sophoridine, an alkaloid compound which is isolated from various plant species like Sophoraalopecuroides and Sophora flavescens. It identified to have potential pharmacological properties, including anti-inflammatory, anticancer and antiviral activities. The effect of the sophoridine on mTOR and NOTCH1 pathways that are critical in NSCLC is not well understood.
Materials and Methods
In order to assess the virtual interaction of mTOR and NOTCH1 with sophoridine, we employed molecular docking. We also performed an in vitro analysis using the NSCLC cell line (A549) using MTT, RT-PCR, western blot and wound healing assays.
Results and Discussion
We report strong interaction of sophoridine with mTOR and it exhibited cytotoxic effect on NSCLC cells, causing downregulation of the mTOR and NOTCH1 genes. We used a wound-healing assay to assess its impact on cell migration in NSCLC cells and our findings confirmed that sophoridine significantly (p = 0.0265) inhibits cell migration in A549 cells.
Conclusion
Our study indicates sophoridine to be potential anti-cancer drug in NSCLC cell via inhibition of mTOR and NOTCH1 axis.
Keywords
Subject
Article metadata
| Title | The Effect of Sophoridine on NSCLC by Down Regulation of mTOR and NOTCH1 |
|---|---|
| Authors | Mohammad Raghibul Hasan; Ali Hazazi; Ahad Amer Alsaiari; Fawaz M. Almufarriji; Amirah Albaqami; Osama Abdulaziz; Marwa K Darwish; Atul Kumar; Kapil Dev; Mohammed Ageeli Hakami |
| Affiliations | Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, SAUDI ARABIA.; Department of Pathology and Laboratory Medicine, Security Forces Hospital Program, Riyadh, SAUDI ARABIA.; College of Medicine, Alfaisal University, Riyadh, SAUDI ARABIA.; Department of Clinical Laboratory Science, College of Applied Medical Science, Taif University, Taif, SAUDI ARABIA.; Department of Clinical Laboratory Sciences, Turabah University College, Taif University, Taif, SAUDI ARABIA.; Department of Chemistry (Biochemistry Branch), Faculty of Science, Suez University, Suez, EGYPT.; Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi, INDIA. |
| Corresponding author | m.hakami@su.edu.sa |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 58, Issue 4 (2024) |
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