Preparation of Topical Nano Gel Loaded With Hesperidin Emulsomes: In vitro and in vivo Studies

Objectives: The objective of the present study was to increase the solubility of poorly soluble drug hesperidin and to increase the efficacy of the drug by loading hesperidin in emulsomal topical gel. Methods: Hesperidin was loaded into emulsomes by using ethanol injection method. The optimized formulation of emulsomes was evaluated by SEM, FT-IR, DSC, P-XRD, in vitro drug release, ex-vivo skin permeation studies, in vivo pharmacokinetic and pharmacodynamic studies. Results: The SEM study shows the emulsomes were tiny and spherical in structure. The particle size and zeta potential of the optimized formulation was found to be 50nm and -1.8mV and in vitro drug release was found to be 98.6% for 6 hrs. The optimized emulgel was prepared by using different gelling agents Carbopol 934 and HPMC. The prepared gels were found to be homogenous and skin permeation studies was found to be 98.9% for 4 hr, with skin permeation efficacy bearing flux value 12.3µg/cm2/h when compared to the pure hesperidin gel. The in vivo pharmacokinetic and pharmacodynamic studies were found to be more efficient for the hesperidin loaded emulsomal gel when compared to the marketed formulation. Conclusion: Hesperidin emulsomal gel has shown a significant increase (p<0.05) in activity when compared to the marketed formulation.