Design and Optimization of Arginine-Loaded Nutraceutical Nanoparticles via Face-Centered Central Composite Approach

Aim/Background

The aim of the present study was to develop and optimize a dosage form of L-arginine as a nanoparticle for prolonged release. The Quality by Design (QbD) approach was employed to develop and optimize the dosage form using Face Centered Central Composite Design (FCCCD). Nutraceutical-loaded nanoparticles were developed by nanoprecipitation technique using Eudragit L 100, methanol and polyvinyl alcohol solution.

Materials and Methods

The concentration of Eudragit L 100 and solvent to antisolvent was considered as independent variable whereas particle size, zeta potential, drug loading and entrapment efficiency were the dependent responses. A 3$^2$ FCCCD was used to develop 3D Response surface plot and to get an optimized formulation.

Results

The optimized formulation demonstrated desirable values of Particle Size of 103.97 nm, Zeta Potential of 0.771, Drug Loading (%) of 24.826, and Entrapment Efficiency (%) 74.26. The optimized formulation was validated by preparing four additional batches using the determined optimal values of independent variables. A comparison between the experimental and predicted values demonstrated close agreement, confirming the reliability of the optimized data.

Conclusion

The current research study helps to optimize L-arginine drug delivery systems, significantly reducing the need for exhaustive experimentation while ensuring high-quality formulations.