LC–MS/MS assay for Acetazolamide, A Carbonic Anhydrase Inhibitor in Human Plasma and its Clinical Application

Narapusetti, Anjaneyulu; Bethanabhatla, Syama Sundar; Sockalingam, Anbazhagan; Pilli, Nageswara Rao

doi:10.5530/jyp.2015.4s.5

Original ArtcileJournal of Young PharmacistsVol. 7 | Issue 4s | pp. 438–445Open access

LC–MS/MS assay for Acetazolamide, A Carbonic Anhydrase Inhibitor in Human Plasma and its Clinical Application

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⁴,
⁵

¹ Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru Technological University Kakinada, Kakinada–533 003, INDIA.
² Department of Pharmaceutical Analysis, Geethanjali College of pharmacy, Cheeryal–501 301, INDIA.
³ Department of Chemistry, Yogi Vemana University, Kadapa–516 003, INDIA.
⁴ Department of Pharmaceutical Chemistry, Surya School of Pharmacy, NH–45, GST Road, Vikravandi, Villpuram–605 652, INDIA.
⁵ Department of Bioanalysis, PCR Laboratories, Ramanthapur, Hyderabad–500 013, INDIA.

Correspondence: Anjaneyulu Narapusetti
Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru Technological University Kakinada, Kakinada–533 003, INDIA.; Department of Pharmaceutical Analysis, Geethanjali College of pharmacy, Cheeryal–501 301, INDIA.
Email: narapusetti@gmail.com

DOI:: 10.5530/jyp.2015.4s.5

How to cite

Narapusetti, A., Bethanabhatla, S. S., Sockalingam, A., & Pilli, N. R. LC–MS/MS assay for Acetazolamide, A Carbonic Anhydrase Inhibitor in Human Plasma and its Clinical Application. Journal of Young Pharmacists, 7(4s), 438–445. https://doi.org/10.5530/jyp.2015.4s.5

Abstract

Objective: The objective of this research was to develop a novel liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of acetazolamide in human plasma. Methods: An analytical method based on LC-MS/MS (API-4000) has been developed and validated for the quantitative determination of acetazolamide in human plasma using acetazolamide d3 as Internal Standard (IS). After Solid phase extraction (SPE), analyte and the IS were chromatographed on a C18 columns using a isocratic mobile phase composed of 0.1% formic acid buffer and acetonitrile (30:70, v/v) pumped at a flow rate of 0.80 mL/min. Results: Precision and accuracy of the method was determined using five analytical batches in the concentration range of 50.3–12046 ng/mL. All the validation experiments were carried out as per the US FDA guidelines and results met the acceptance criteria. Conclusion: The proposed LC–MS/MS assay method is simple, rapid and sensitive for the determination of acetazolamide in human plasma. A chromatographic run time of 2.0 min, allow us to analyze more than 300 samples in a day.

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Article metadata

Title	LC–MS/MS assay for Acetazolamide, A Carbonic Anhydrase Inhibitor in Human Plasma and its Clinical Application
Authors	Anjaneyulu Narapusetti; Syama Sundar Bethanabhatla; Anbazhagan Sockalingam; Nageswara Rao Pilli
Affiliations	Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru Technological University Kakinada, Kakinada–533 003, INDIA.; Department of Pharmaceutical Analysis, Geethanjali College of pharmacy, Cheeryal–501 301, INDIA.; Department of Chemistry, Yogi Vemana University, Kadapa–516 003, INDIA.; Department of Pharmaceutical Chemistry, Surya School of Pharmacy, NH–45, GST Road, Vikravandi, Villpuram–605 652, INDIA.; Department of Bioanalysis, PCR Laboratories, Ramanthapur, Hyderabad–500 013, INDIA.
Corresponding author	narapusetti@gmail.com
Journal	Journal of Young Pharmacists
Volume / Issue	Vol. 7, Issue 4s

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