Phytochemical screening, Antioxidant, Thrombolytic, α-amylase inhibition and cytotoxic activities of ethanol extract of Steudnera colocasiifolia K. Koch leaves

Objective: Present study aims to investigate both qualitative and quantitative phytochemicals presence, antioxidant, thrombolytic, α-amylase and cytotoxic activity of a Bangladeshi plant Steudnera colocasiifolia K. Koch leaf ethanolic extract. Methods: Phytochemical screening was accomplished by using established methods. Thrombolytic assay was conducted through clot lysis method. Alpha amylase inhibition assay was made by modified enzyme inhibitory action and cytotoxicity was studied by brine shrimp lethality test. Data was analyzed by One Way Analysis of Variance (ANOVA) using statistical software Statistical Package for Social Science (SPSS, Version 22.0, IBM corporation, NY) followed by student ‘t’ test with GraphPad Prism Data Editor for Windows, Version 6.0 (GraphPad software Inc., San Diego, CA). Results: Primary screening shows the presence of major metabolites such as alkaloid, flavonoid, tannin, saponins, steroid, quinone, cellulose and glycosides while the quantitative analysis elicits the presence of magnificent amount of flavonoid (70.60 ± 0.23), proanthocyanidin (64.46 ± 0.58), flavonol (71.22 ± 0.07) and total antioxidant (66.40 ± 0.26) in the extract. The thrombolytic effect of S. colocasiifolia was found to be 35.16% and this extract had good α-amylase inhibitory activity (IC50=2.16 ± 0.06 mg/ml) as compared to Acarbose. The extract was found to have a LC50 value of 305.2 μg/ml in Brine shrimp lethality bioassay. Conclusion: Results demonstrate the Steudnera colocasiifolia has a very good prospect to be studied further for its extended antioxidative and α-amylase inhibitory effects.