Original ArticleFree Radicals and AntioxidantsVol. 3 | Issue 2 | pp. 100–106Open access
Evaluation of antioxidant and DNA nicking potential along with HPTLC fingerprint analysis of different parts of Pterospermum acerifolium (L.) Willd
- 1,
- 1*,
- 1,
- 2,
- 2
- 1 Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, Jharkhand, India.
- 2 Department of Biotechnology, Birla Institute of Technology, Ranchi 835215, Jharkhand, India.
Published in Free Radicals and Antioxidants
Correspondence: Papiya Mitra Mazumder
Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, Jharkhand, India.
Email: pmitramazumder@bitmesra.ac.in
- Received:
- Mar 15, 2013
- Accepted:
- Aug 22, 2013
- DOI:
- 10.1016/j.fra.2013.05.002
How to cite
Paramaguru, R., Mazumder, P. M., Sasmal, D., Kumar, D., & Mukhopadhyay, K. Evaluation of antioxidant and DNA nicking potential along with HPTLC fingerprint analysis of different parts of Pterospermum acerifolium (L.) Willd. Free Radicals and Antioxidants, 3(2), 100–106. https://doi.org/10.1016/j.fra.2013.05.002
Abstract
Objective: The present study was designed to evaluate the in vitro antioxidant and DNA nicking potential of various extracts and fractions of Pterospermum acerifolium (L.) Willd (Family: Stericuliaceae). Research design and methods: Antioxidant properties of the extracts and fractions was assayed by DPPH scavenging activity, non-site-specific and site-specific OH radical scavenging activity mediated 2-deoxy- D-ribose degradation, total antioxidant activity and lipid peroxidation assay by using rat liver homoge- nate. DNA nicking assay was studied by PT257R/T plasmid. Estimation of total phenolic content and total flavonoid content was done. Further HPTLC fingerprint of active fractions was performed. Results: Ethyl acetate fractions of leaves, flowers and bark exhibited potent antioxidant property and DNA protective effect compare to all the other extracts and fractions. Total phenolic and flavonoid content determination was also showed ethyl acetate fractions were rich in phenolic and flavonoid contents. HPTLC fingerprint revealed the total number of peaks present in the active ethyl acetate fractions of leaves, flowers and bark. Conclusion: The present study indicated that, ethyl acetate fractions of leaves, flowers and bark showed effective antioxidant and DNA protection activity and it could be the initiation for various other phar- macological studies on those fractions.
Keywords
Subject
Article metadata
| Title | Evaluation of antioxidant and DNA nicking potential along with HPTLC fingerprint analysis of different parts of Pterospermum acerifolium (L.) Willd |
|---|---|
| Authors | Rathinavelusamy Paramaguru; Papiya Mitra Mazumder; Dinakar Sasmal; Dhananjay Kumar; Kunal Mukhopadhyay |
| Affiliations | Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, Jharkhand, India.; Department of Biotechnology, Birla Institute of Technology, Ranchi 835215, Jharkhand, India. |
| Corresponding author | pmitramazumder@bitmesra.ac.in |
| Journal | Free Radicals and Antioxidants |
| Volume / Issue | Vol. 3, Issue 2 |
Also in this issue
- Antioxidant and DNA damage protecting activities of Eulophia nuda Lindl.pp. 55–60
- Spinning, oxidative damage and hemolysis in athletespp. 61–66
- Studies on antioxidants and peroxidase isoenzymes in seedlings of twelve cultivars with four different durations of flowering time in pigeon pea (Cajanus cajan (L.) Millspaugh)pp. 67–72
- Hepatoprotective mechanisms of Ageratum conyzoides L. on oxidative damage induced by acetaminophen in Wistar ratspp. 73–76
- Phytochemical constituents and in vitro antioxidant capacity of Tabernaemontana catharinensis A. DCpp. 77–80